验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse testis tissue |
For other applications, we recommend the unconjugated version of this antibody, 15697-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-15697 targets PRM1 in IF-P applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag8323 Product name: Recombinant human PRM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-51 aa of BC003673 Sequence: MARYRCCRSQSRSRYYRQRQRSRRRRRRSCQTRRRAMRCCRPRYRPRCRR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PRM1 |
| 别名 | P1, Cysteine-rich protamine, CT94.1 |
| 计算分子量 | 51 aa, 6 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC003673 |
| 基因名称 | PRM1 |
| Gene ID (NCBI) | 5619 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity Purified |
| UNIPROT ID | P04553 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRM1 is a 51 amino acid protein. PRM1 substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823 )
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 PRM1 antibody CL594-15697 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
