验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 1 publications below |
产品信息
15697-1-AP targets PRM1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8323 Product name: Recombinant human PRM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-51 aa of BC003673 Sequence: MARYRCCRSQSRSRYYRQRQRSRRRRRRSCQTRRRAMRCCRPRYRPRCRR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PRM1 |
| 别名 | P1, Cysteine-rich protamine, CT94.1 |
| 计算分子量 | 51 aa, 6 kDa |
| 观测分子量 | 13-20 kDa |
| GenBank蛋白编号 | BC003673 |
| 基因名称 | PRM1 |
| Gene ID (NCBI) | 5619 |
| RRID | AB_2878168 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04553 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRM1 is a 51 amino acid protein. PRM1 substitutes for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRM1 antibody 15697-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Environ Pollut Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice. | ||
Theriogenology Eif2s3y alleviated LPS-induced damage to mouse testis and maintained spermatogenesis by negatively regulating Adamts5 | ||
Reprod Sci Sertoli Cell-Conditioned Medium Induces Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells to Male Germ-Like Cells in Busulfan-Induced Azoospermic Mouse Model | ||
Eur J Pharmacol 5- methylcytidine effectively improves spermatogenesis recovery in busulfan-induced oligoasthenospermia mice | ||
Cell Rep Med Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling |
