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验证数据展示

  • WB analysis using 16794-1-AP (same clone as 16794-1-PBS)

    WB analysis using 16794-1-AP (same clone as 16794-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16794-1-AP (PRKRIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

  • WB analysis using 16794-1-AP (same clone as 16794-1-PBS)

    WB analysis using 16794-1-AP (same clone as 16794-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16794-1-AP (PRKRIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 16794-1-AP (same clone as 16794-1-PBS)

    IP experiment of NIH/3T3 using 16794-1-AP (same clone as 16794-1-PBS)

    IP result of anti-PRKRIP1 (IP:16794-1-AP, 4ug; Detection:16794-1-AP 1:2000) with NIH/3T3 cells lysate 1200 ug. This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 16794-1-AP (same clone as 16794-1-PBS)

    IHC staining of human colon cancer using 16794-1-AP (same clone as 16794-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16794-1-AP (PRKRIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

  • IF Staining of Caco-2 using 16794-1-AP (same clone as 16794-1-PBS)

    IF Staining of Caco-2 using 16794-1-AP (same clone as 16794-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PRKRIP1 antibody (16794-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

PRKRIP1 Polyclonal antibody, PBS Only

PRKRIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16794-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

C114, PRKR interacting protein 1, PRKR-interacting protein 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16794-1-PBS targets PRKRIP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10266

Product name: Recombinant human PRKRIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-184 aa of BC014298

Sequence: MASPAASSVRPPRPKKEPQTLVIPKNAAEEQKLKLERLMKNPDKAVPIPEKMSEWAPRPPPEFVRDVMGSSAGAGSGEFHVYRHLRRREYQRQDYMDAMAEKQKLYAEFQKRLEKNKIAAEEQTAKRRKKRQKLKEKKLLAKKMKLEQKKQEGPGQPKEQGSSSSAEASGTEEEEEVPSFTMGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PRKR interacting protein 1 (IL11 inducible)
别名 C114, PRKR interacting protein 1, PRKR-interacting protein 1
计算分子量 184 aa, 21 kDa
观测分子量21-25 kDa
GenBank蛋白编号BC014298
基因名称 PRKRIP1
Gene ID (NCBI) 79706
RRIDAB_3741894
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H875
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRKR-interacting protein 1 (PRKRIP1), a component of the splicing C complex, is a double-stranded RNA-binding protein. PRKRIP1 consists of a fully extended N-terminal loop (residues 51-75) and an 18-turn α helix (residues 76-142) that spans 100 Å (PMID: 28502770). The full-length cDNA contains an open reading frame of 561 bp, which encodes an 184-amino acid polypeptide with a predicted molecular mass of 21 kDa (PMID: 12679338).

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