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验证数据展示

  • WB analysis using 16794-1-AP

    WB analysis using 16794-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16794-1-AP (PRKRIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of NIH/3T3 using 16794-1-AP

    IP experiment of NIH/3T3 using 16794-1-AP

    IP result of anti-PRKRIP1 (IP:16794-1-AP, 4ug; Detection:16794-1-AP 1:2000) with NIH/3T3 cells lysate 1200 ug.

  • IHC staining of human colon cancer using 16794-1-AP

    IHC staining of human colon cancer using 16794-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16794-1-AP (PRKRIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Caco-2 using 16794-1-AP

    IF Staining of Caco-2 using 16794-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PRKRIP1 antibody (16794-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

PRKRIP1 Polyclonal antibody

PRKRIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16794-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

C114, PRKR interacting protein 1, PRKR-interacting protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16794-1-AP targets PRKRIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 PRKRIP1 fusion protein Ag10266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PRKR interacting protein 1 (IL11 inducible)
别名 C114, PRKR interacting protein 1, PRKR-interacting protein 1
计算分子量 184 aa, 21 kDa
观测分子量21-25 kDa
GenBank蛋白编号BC014298
基因名称 PRKRIP1
Gene ID (NCBI) 79706
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H875
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRKR-interacting protein 1 (PRKRIP1), a component of the splicing C complex, is a double-stranded RNA-binding protein. PRKRIP1 consists of a fully extended N-terminal loop (residues 51-75) and an 18-turn α helix (residues 76-142) that spans 100 Å (PMID: 28502770). The full-length cDNA contains an open reading frame of 561 bp, which encodes an 184-amino acid polypeptide with a predicted molecular mass of 21 kDa (PMID: 12679338).

实验方案

Product Specific Protocols
WB protocol for PRKRIP1 antibody 16794-1-APDownload protocol
IHC protocol for PRKRIP1 antibody 16794-1-APDownload protocol
IF protocol for PRKRIP1 antibody 16794-1-APDownload protocol
IP protocol for PRKRIP1 antibody 16794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols