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验证数据展示

  • WB analysis of HEK-293 using 10860-1-AP

    WB analysis of HEK-293 using 10860-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10860-1-AP (PKC zeta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10860-1-AP

    IHC staining of human breast cancer using 10860-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human breast cancer using 10860-1-AP

    IHC staining of human breast cancer using 10860-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse cerebellum using 10860-1-AP

    IHC staining of mouse cerebellum using 10860-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10860-1-AP (PKC Zeta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 10860-1-AP

    IHC staining of mouse cerebellum using 10860-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10860-1-AP (PKC Zeta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 10860-1-AP

    IHC staining of mouse eye using 10860-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10860-1-AP (PKC Zeta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 10860-1-AP

    IHC staining of mouse eye using 10860-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 10860-1-AP (PKC Zeta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 10860-1-AP

    IF Staining of NIH/3T3 using 10860-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PKC Zeta antibody (10860-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

PKC Zeta Polyclonal antibody

PKC Zeta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10860-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

PRKCZ, Protein kinase C zeta type, PKC2, nPKC-zeta, EC:2.7.11.13

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, mouse cerebellum tissue, mouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10860-1-AP targets PKC Zeta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag1310

Product name: Recombinant human PRKCZ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 244-592 aa of BC014270

Sequence: SQGLGLQDFDLIRVIGRGSYAKVLLVRLKKNDQIYAMKVVKKELVHDDEDIDWVQTEKHVFEQASSNPFLVGLHSCFQTTSRLFLVIEYVNGGDLMFHMQRQRKLPEEHARFYAAEICIALNFLHERGIIYRDLKLDNVLLDADGHIKLTDYGMCKEGLGPGDTTSTFCGTPNYIAPEILRGEEYGFSVDWWALGVLMFEMMAGRSPFDIITDNPDMNTEDYLFQVILEKPIRIPRFLSVKASHVLKGFLNKDPKERLGCRPQTGFSDIKSHAFFRSIDWDLLEKKQALPPFQPQITDDYGLDNFDTQFTSEPVQLTPDDEDAIKRIDQSEFEGFEYINPLLLSTEESV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase C, zeta
别名 PRKCZ, Protein kinase C zeta type, PKC2, nPKC-zeta, EC:2.7.11.13
计算分子量 80 kDa
观测分子量 68 kDa
GenBank蛋白编号BC014270
基因名称 PKC Zeta
Gene ID (NCBI) 5590
RRIDAB_2300348
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05513
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for PKC Zeta antibody 10860-1-APDownload protocol
IHC protocol for PKC Zeta antibody 10860-1-APDownload protocol
WB protocol for PKC Zeta antibody 10860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Sci Rep

aPKCζ-dependent Repression of Yap is Necessary for Functional Restoration of Irradiated Salivary Glands with IGF-1.

Authors - Alejandro M Chibly
  • KO Validated
View Article
human,mouseWB,IHC

Cancers (Basel)

Hypermethylation of PRKCZ Regulated by E6 Inhibits Invasion and EMT via Cdc42 in HPV-Related Head and Neck Squamous Cell Carcinoma

Authors - Hao-Fan Wang
  • KD Validated
View Article
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