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  • Featured Product
  • KD/KO Validated

PKC Zeta Polyclonal antibody

PKC Zeta Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10860-1-AP

Print datasheet

Synonyms

PKC ZETA, PKC2, PRKCZ, Protein kinase C zeta type, protein kinase C, zeta

验证数据展示

  • WB analysis of HEK-293 using 10860-1-AP

    WB analysis of HEK-293 using 10860-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10860-1-AP (PKC zeta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10860-1-AP

    IHC staining of human breast cancer using 10860-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human breast cancer using 10860-1-AP

    IHC staining of human breast cancer using 10860-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10860-1-AP targets PKC Zeta in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen PKC Zeta fusion protein Ag1310 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase C, zeta
Synonyms PKC ZETA, PKC2, PRKCZ, Protein kinase C zeta type, protein kinase C, zeta
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC014270
Gene Symbol PRKCZ
Gene ID (NCBI) 5590
RRIDAB_2300348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05513
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PKC Zeta antibody 10860-1-APDownload protocol
IHC protocol for PKC Zeta antibody 10860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Sci Rep

aPKCζ-dependent Repression of Yap is Necessary for Functional Restoration of Irradiated Salivary Glands with IGF-1.

Authors - Alejandro M Chibly
  • KO Validated
View Article
human,mouseWB,IHC

Cancers (Basel)

Hypermethylation of PRKCZ Regulated by E6 Inhibits Invasion and EMT via Cdc42 in HPV-Related Head and Neck Squamous Cell Carcinoma

Authors - Hao-Fan Wang
  • KD Validated
View Article