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验证数据展示

  • WB analysis of HeLa using 16250-1-AP (same clone as 16250-1-PBS)

    WB analysis of HeLa using 16250-1-AP (same clone as 16250-1-PBS)

    WB result of PRKCDBP antibody (16250-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKCDBP transfected HeLa cells. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

  • WB analysis using 16250-1-AP (same clone as 16250-1-PBS)

    WB analysis using 16250-1-AP (same clone as 16250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 16250-1-AP (same clone as 16250-1-PBS)

    IF Staining of MCF-7 using 16250-1-AP (same clone as 16250-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using PRKCDBP antibody 16250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

  • IP experiment of A549 using 16250-1-AP (same clone as 16250-1-PBS)

    IP experiment of A549 using 16250-1-AP (same clone as 16250-1-PBS)

    IP result of anti-PRKCDBP (IP:16250-1-AP, 4ug; Detection:16250-1-AP 1:1000) with A549 cells lysate 1120 ug. This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 16250-1-AP (same clone as 16250-1-PBS)

    IHC staining of human colon using 16250-1-AP (same clone as 16250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16250-1-AP (PRKCDBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCDBP/CAVIN3 antibody included
  • Featured Product
  • KD/KO Validated

PRKCDBP Polyclonal antibody, PBS Only

PRKCDBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16250-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Caveolae-associated protein 3, Cavin 3, Cavin-3, hSRBC, Protein kinase C delta-binding protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRKCDBP/CAVIN3 Ready-To-Use IHC Kit. PRKCDBP/CAVIN3 primary antibody included.

产品信息

16250-1-PBS targets PRKCDBP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9209

Product name: Recombinant human PRKCDBP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-261 aa of BC011585

Sequence: MRESALEPGPVPEAPAGGPVHAVTVVTLLEKLASMLETLRERQGGLARRQGGLAGSVRRIQSGLGALSRSHDTTSNTLAQLLAKAERVSSHANAAQERAVRRAAQVQRLEANHGLLVARGKLHVLLFKEEGEVPASAFQKAPEPLGPADQSELGPEQLEAEVGESSDEEPVESRAQRLRRTGLQKVQSLRRALSGRKGPAAPPPTPVKPPRLGPGRSAEAQPEAQPALEPTLEPEPPQDTEEDPGRPGAAEEALLQMESVA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase C, delta binding protein
别名 Caveolae-associated protein 3, Cavin 3, Cavin-3, hSRBC, Protein kinase C delta-binding protein
计算分子量 261 aa, 28 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC011585
基因名称 PRKCDBP
Gene ID (NCBI) 112464
RRIDAB_2171894
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969G5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein kinase C delta-binding protein (PRKCDBP) is a putative tumor suppressor in which alteration has been observed in several human cancers. It is a BRCA1-interacting protein, and may also be involved in DNA damage response and participate in BRCA1-mediated tumor suppression pathways. PKCdelta binds to the C-terminal half of PRKCDBP through the regulatory domain and phosphorylates it in vitro. In COS1 cells, the phosphorylation of over-expressed PRKCDBP is stimulated by 12-O-tetradecanoylphorbol-13-acetate and further enhanced by the over-expression of PKCdelta

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