- Featured Product
- KD/KO Validated
PRKAR2A Polyclonal antibody
PRKAR2A Polyclonal Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, K-562 cells, MCF-7 cells, mouse testis tissue, PC-3 cells, human testis tissue |
Positive IP detected in | HeLa cells, HeLa cells |
Positive IF detected in | HeLa cells, HepG2 cells, MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10142-2-AP targets PRKAR2A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IP, WB, ELISA |
Cited Applications | WB, IP, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | PRKAR2A fusion protein Ag0203 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein kinase, cAMP-dependent, regulatory, type II, alpha |
Synonyms | PKR2, PRKAR2, PRKAR2A |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 46-52 kDa |
GenBank Accession Number | BC002763 |
Gene Symbol | PRKAR2A |
Gene ID (NCBI) | 5576 |
RRID | AB_2168236 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P13861 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PRKAR2A antibody 10142-2-AP | Download protocol |
IF protocol for PRKAR2A antibody 10142-2-AP | Download protocol |
IP protocol for PRKAR2A antibody 10142-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Lett Depletion of LAMP3 enhances PKA-mediated VASP phosphorylation to suppress invasion and metastasis in esophageal squamous cell carcinoma.
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Life Sci TMT-based proteomics analysis to screen potential biomarkers of acute-phase TBI in rats. | ||