验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PRKACB Ready-To-Use IHC Kit. PRKACB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12232-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-12232 targets PRKACB in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PRKACB fusion protein Ag2870 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase, cAMP-dependent, catalytic, beta |
| 别名 | DKFZp781I2452, PKA C beta, PKACB, PRKACB |
| 计算分子量 | 41 kDa, 46 kDa |
| 观测分子量 | 36-55 kDa |
| GenBank蛋白编号 | BC016285 |
| 基因名称 | PRKACB |
| Gene ID (NCBI) | 5567 |
| RRID | AB_3672529 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P22694 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PRKACB antibody CL488-12232 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |