验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, fetal human brain tissue |
Planning an IHC experiment? We recommend our IHCeasy PRKACB Ready-To-Use IHC Kit. PRKACB primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85716-2-RR targets PRKACB in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein kinase, cAMP-dependent, catalytic, beta |
| 别名 | EC:2.7.11.11, PKA C beta, PKA C-beta, PKACB |
| 计算分子量 | 46 kDa |
| 观测分子量 | 36-46 kDa |
| GenBank蛋白编号 | NM_002731 |
| 基因名称 | PRKACB |
| Gene ID (NCBI) | 5567 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P22694 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRKACB antibody 85716-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
