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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PRICKLE1 Polyclonal antibody

PRICKLE1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-22589

Print datasheet

Synonyms

EPM1B, prickle homolog 1 (Drosophila), Prickle like protein 1, PRICKLE1, RILP

验证数据展示

  • FC experiment of HeLa using CL488-22589

    FC experiment of HeLa using CL488-22589

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PRICKLE1 (CL488-22589)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRICKLE1 Ready-To-Use IHC Kit. PRICKLE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22589-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22589 targets PRICKLE1 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse
Immunogen PRICKLE1 fusion protein Ag18407 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prickle homolog 1 (Drosophila)
Synonyms EPM1B, prickle homolog 1 (Drosophila), Prickle like protein 1, PRICKLE1, RILP
Calculated Molecular Weight 831 aa, 94 kDa
Observed Molecular Weight 94 kDa, 55-60 kDa
GenBank Accession NumberBC114939
Gene Symbol PRICKLE1
Gene ID (NCBI) 144165
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MT3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PRICKLE1 antibody CL488-22589Download protocol
Standard Protocols
Click here to view our Standard Protocols