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验证数据展示

  • WB analysis using 23783-1-AP

    WB analysis using 23783-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23783-1-AP (PRELP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 23783-1-AP

    IHC staining of human stomach cancer using 23783-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23783-1-AP (PRELP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 23783-1-AP

    IF Staining of U2OS using 23783-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PRELP antibody (23783-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

PRELP Polyclonal antibody

PRELP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23783-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

SLRR2A, Proline-arginine-rich end leucine-rich repeat protein, Prolargin, MSTP161, MST161

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, SMMC-7721 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23783-1-AP targets PRELP in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag20656

Product name: Recombinant human PRELP protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 20-361 aa of BC032498

Sequence: GQPTRRPRPGTGPGRRPRPRPRPTPSFPQPDEPAEPTDLPPPLPPGPPSIFPDCPRECYCPPDFPSALYCDSRNLRKVPVIPPRIHYLYLQNNFITELPVESFQNATGLRWINLDNNRIRKIDQRVLEKLPGLVFLYMEKNQLEEVPSALPRNLEQLRLSQNHISRIPPGVFSKLENLLLLDLQHNRLSDGVFKPDTFHGLKNLMQLNLAHNILRKMPPRVPTAIHQLYLDSNKIETIPNGYFKSFPNLAFIRLNYNKLTDRGLPKNSFNISNLLVLHLSHNRISSVPAINNRLEHLYLNNNSIEKINGTQICPNDLVAFHDFSSDLENVPHLRYLRLDGNY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proline/arginine-rich end leucine-rich repeat protein
别名 SLRR2A, Proline-arginine-rich end leucine-rich repeat protein, Prolargin, MSTP161, MST161
计算分子量 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC032498
基因名称 PRELP
Gene ID (NCBI) 5549
RRIDAB_3085714
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51888
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRELP (proline/arginine-rich end leucine-rich repeat protein), also known as MST161, MSTP161, prolargin, or SLRR2A, belongs to the class II subfamily of the small leucine-rich proteoglycan (SLRP) family. PRELP is a small leucine-rich proteoglycan (SLRP), which binds collagen to basement membranes or cartilage as a matrix protein. PRELP has prognostic value and regulates cell proliferation and migration in hepatocellular carcinoma, may serve as a potential prognostic marker and a treatment target against HCC progression (PMID: 33033521).

实验方案

Product Specific Protocols
WB protocol for PRELP antibody 23783-1-APDownload protocol
IHC protocol for PRELP antibody 23783-1-APDownload protocol
IF protocol for PRELP antibody 23783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,CoIP

Apoptosis

PRELP inhibits colorectal cancer progression by suppressing epithelial-mesenchymal transition and angiogenesis via the inactivation of the FGF1/PI3K/AKT pathway

Authors - Xiaoqing Li
  • KD Validated
View Article
humanWB,IHC

Front Med (Lausanne)

Investigating the role of disulfidptosis related genes in radiotherapy resistance of lung adenocarcinoma

Authors - Xiaoxia Pan
View Article