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PRELP Polyclonal antibody

PRELP Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23783-1-AP

Print datasheet

Synonyms

MST161, MSTP161, PRELP, Prolargin, SLRR2A

验证数据展示

  • WB analysis using 23783-1-AP

    WB analysis using 23783-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23783-1-AP (PRELP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 23783-1-AP

    IF Staining of U2OS using 23783-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PRELP antibody (23783-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells, SMMC-7721 cells
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23783-1-AP targets PRELP in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen PRELP fusion protein Ag20656 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proline/arginine-rich end leucine-rich repeat protein
Synonyms MST161, MSTP161, PRELP, Prolargin, SLRR2A
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC032498
Gene Symbol PRELP
Gene ID (NCBI) 5549
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51888
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRELP (proline/arginine-rich end leucine-rich repeat protein), also known as MST161, MSTP161, prolargin, or SLRR2A, belongs to the class II subfamily of the small leucine-rich proteoglycan (SLRP) family. PRELP is a small leucine-rich proteoglycan (SLRP), which binds collagen to basement membranes or cartilage as a matrix protein. PRELP has prognostic value and regulates cell proliferation and migration in hepatocellular carcinoma, may serve as a potential prognostic marker and a treatment target against HCC progression (PMID: 33033521).

实验方案

Product Specific Protocols
WB protocol for PRELP antibody 23783-1-APDownload protocol
IF protocol for PRELP antibody 23783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols