验证数据展示
经过测试的应用
Positive WB detected in | L02 cells, SMMC-7721 cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23783-1-AP targets PRELP in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, CoIP |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | PRELP fusion protein Ag20656 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | proline/arginine-rich end leucine-rich repeat protein |
别名 | SLRR2A, Proline-arginine-rich end leucine-rich repeat protein, Prolargin, MSTP161, MST161 |
计算分子量 | 44 kDa |
观测分子量 | 44 kDa |
GenBank蛋白编号 | BC032498 |
基因名称 | PRELP |
Gene ID (NCBI) | 5549 |
RRID | AB_3085714 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P51888 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRELP (proline/arginine-rich end leucine-rich repeat protein), also known as MST161, MSTP161, prolargin, or SLRR2A, belongs to the class II subfamily of the small leucine-rich proteoglycan (SLRP) family. PRELP is a small leucine-rich proteoglycan (SLRP), which binds collagen to basement membranes or cartilage as a matrix protein. PRELP has prognostic value and regulates cell proliferation and migration in hepatocellular carcinoma, may serve as a potential prognostic marker and a treatment target against HCC progression (PMID: 33033521).
实验方案
Product Specific Protocols | |
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WB protocol for PRELP antibody 23783-1-AP | Download protocol |
IHC protocol for PRELP antibody 23783-1-AP | Download protocol |
IF protocol for PRELP antibody 23783-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Apoptosis PRELP inhibits colorectal cancer progression by suppressing epithelial-mesenchymal transition and angiogenesis via the inactivation of the FGF1/PI3K/AKT pathway
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Front Med (Lausanne) Investigating the role of disulfidptosis related genes in radiotherapy resistance of lung adenocarcinoma |