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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PRDX6 Polyclonal antibody

PRDX6 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-13585

Print datasheet

Synonyms

1 Cys, 1 Cys peroxiredoxin, 1 Cys PRX, 24 kDa protein, aiPLA2, Antioxidant protein 2, AOP2, KIAA0106, Liver 2D page spot 40, NSGPx, p29, peroxiredoxin 6, PRDX6, PRX, Red blood cells page spot 12

验证数据展示

  • IF Staining of HeLa using CL488-13585

    IF Staining of HeLa using CL488-13585

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PRDX6 antibody (CL488-13585) at dilution of 1:500.

  • FC experiment of HeLa using CL488-13585

    FC experiment of HeLa using CL488-13585

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PRDX6 (CL488-13585) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRDX6 Ready-To-Use IHC Kit. PRDX6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13585-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13585 targets PRDX6 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen PRDX6 fusion protein Ag4512 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peroxiredoxin 6
Synonyms 1 Cys, 1 Cys peroxiredoxin, 1 Cys PRX, 24 kDa protein, aiPLA2, Antioxidant protein 2, AOP2, KIAA0106, Liver 2D page spot 40, NSGPx, p29, peroxiredoxin 6, PRDX6, PRX, Red blood cells page spot 12
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC035857
Gene Symbol PRDX6
Gene ID (NCBI) 9588
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30041
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PRDX6 antibody CL488-13585Download protocol
FC protocol for CL Plus 488 PRDX6 antibody CL488-13585Download protocol
Standard Protocols
Click here to view our Standard Protocols