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验证数据展示

  • IF Staining of rat pancreas using 15493-1-AP

    IF Staining of rat pancreas using 15493-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat pancreas tissue using Pancreatic Polypeptide antibody (15493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Pancreatic Polypeptide antibody (15493-1-AP, green) stained on PP cells in rat pancreas islet. Glucagon antibody (67286-1-Ig, Clone: 2A8G1, red) stained on α cells in rat pancreas islet.

  • IF Staining of rat pancreas using 15493-1-AP

    IF Staining of rat pancreas using 15493-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat pancreas tissue using Pancreatic Polypeptide antibody (15493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Pancreatic Polypeptide antibody (15493-1-AP) (green) stained on PP cells in rat pancreas islet. Glucagon antibody (67286-1-Ig, Clone: 2A8G1, red) stained on α cells in rat pancreas islet.

  • IHC staining of mouse pancreas using 15493-1-AP

    IHC staining of mouse pancreas using 15493-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15493-1-AP (Pancreatic Polypeptide antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 15493-1-AP

    IHC staining of mouse pancreas using 15493-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15493-1-AP (Pancreatic Polypeptide antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 15493-1-AP

    FC experiment of HepG2 using 15493-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Pancreatic Polypeptide (15493-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Pancreatic Polypeptide Polyclonal antibody

Pancreatic Polypeptide Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA
Cat No. 15493-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, FC (Intra), ELISA and More (1)

别名

PPY, Obinepitide, Pancreatic icosapeptide, Pancreatic polypeptide prohormone, Pancreatic polypeptide Y

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat pancreas tissue
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15493-1-AP targets Pancreatic Polypeptide in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, FC (Intra), ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag7886

Product name: Recombinant human PPY protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-95 aa of BC032225

Sequence: MAAARLCLSLLLLSTCVALLLQPLLGAQGAPLEPVYPGDNATPEQMAQYAADLRRYINMLTRPRYGKRHKEDTLAFSEWGSPHAAVPRELSPLDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pancreatic polypeptide
别名 PPY, Obinepitide, Pancreatic icosapeptide, Pancreatic polypeptide prohormone, Pancreatic polypeptide Y
计算分子量 10 kDa
GenBank蛋白编号BC032225
基因名称 Pancreatic Polypeptide
Gene ID (NCBI) 5539
RRIDAB_2878145
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01298
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for Pancreatic Polypeptide antibody 15493-1-APDownload protocol
IF protocol for Pancreatic Polypeptide antibody 15493-1-APDownload protocol
IHC protocol for Pancreatic Polypeptide antibody 15493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIF

Adv Sci (Weinh)

PPY-Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer

Authors - Mengdie Cao
View Article
mouseIHC

Transpl Int

Formation of Re-Aggregated Neonatal Porcine Islet Clusters Improves In Vitro Function and Transplantation Outcome

Authors - M Honarpisheh
View Article
IF

Vet Med Sci

Histology and immunofluorescent study of the pancreas in lovebird (Agapornis personatus)

Authors - Nader Goodarzi
View Article
mouseIF

Diabetes

Mettl3-Mediated m6A Methylation Controls Pancreatic Bipotent Progenitor Fate and Islet Formation

Authors - Jiajun Sun
View Article

Cell Rep

A ONECUT1 regulatory, non-coding region in pancreatic development and diabetes

Authors - Sarah Merz
View Article
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