验证数据展示
经过测试的应用
| Positive WB detected in | Sp2/0 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
17106-1-AP targets PPWD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag10785 Product name: Recombinant human PPWD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 296-646 aa of BC015385 Sequence: ATIGSDRKVRIFRFVTGKLMRVFDESLSMFTELQQMRQQLPDMEFGRRMAVERELEKVDAVRLINIVFDETGHFVLYGTMLGIKVINVETNRCVRILGKQENIRVMQLALFQGIAKKHRAATTIEMKASENPVLQNIQADPTIVCTSFKKNRFYMFTKREPEDTKSADSDRDVFNEKPSKEEVMAATQAEGPKRVSDSAIIHTSMGDIHTKLFPVECPKTVENFCVHSRNGYYNGHTFHRIIKGFMIQTGDPTGTGMGGESIWGGEFEDEFHSTLRHDRPYTLSMANAGSNTNGSQFFITVVPTPWLDNKHTVFGRVTKGMEVVQRISNVKVNPKTDKPYEDVSIINITVK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peptidylprolyl isomerase domain and WD repeat containing 1 |
| 别名 | KIAA0073, PPWD1 |
| 计算分子量 | 646 aa, 74 kDa |
| 观测分子量 | 65-66 kDa |
| GenBank蛋白编号 | BC015385 |
| 基因名称 | PPWD1 |
| Gene ID (NCBI) | 23398 |
| RRID | AB_2237509 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96BP3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Peptidylprolyl isomerase domain and WD repeat-containing protein1 belongs to cyclophilin family.PPWD1 accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.This antibody recognizes the C-terminal of PPWD1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPWD1 antibody 17106-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
