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验证数据展示

  • WB analysis using 87902-1-RR

    WB analysis using 87902-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87902-1-RR (PPP3CA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 87902-1-RR

    IHC staining of human heart using 87902-1-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 87902-1-RR (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP3CA antibody included
  • IHC staining of human heart using 87902-1-RR

    IHC staining of human heart using 87902-1-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 87902-1-RR (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP3CA antibody included
  • IF Staining of SH-SY5Y using 87902-1-RR

    IF Staining of SH-SY5Y using 87902-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP3CA antibody (87902-1-RR, Clone: 253412F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87902-1-RR

    Affinity of 87902-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87902-1-RR against Human PPP3CA were performed. The affinity constant is below 1 pM.

PPP3CA Recombinant monoclonal antibody

PPP3CA Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 87902-1-RR

产品说明书

CloneNo. 253412F1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CALNA, Calcineurin A alpha, Calmodulin-dependent calcineurin A subunit alpha isoform, CALN, CALNA1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, SH-SY5Y cells, NIH/3T3 cells, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy PPP3CA Ready-To-Use IHC Kit. PPP3CA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87902-1-RR targets PPP3CA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4203

Product name: Recombinant human PPP3CA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3-302 aa of BC025714

Sequence: EPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
别名 CALNA, Calcineurin A alpha, Calmodulin-dependent calcineurin A subunit alpha isoform, CALN, CALNA1
计算分子量 59 kDa
观测分子量 59 kDa
GenBank蛋白编号BC025714
基因名称 PPP3CA
Gene ID (NCBI) 5530
RRIDAB_3745817
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ08209
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.

实验方案

Product Specific Protocols
IF protocol for PPP3CA antibody 87902-1-RRDownload protocol
IHC protocol for PPP3CA antibody 87902-1-RRDownload protocol
WB protocol for PPP3CA antibody 87902-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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