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验证数据展示

  • WB analysis of HeLa using 23885-1-AP

    WB analysis of HeLa using 23885-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23885-1-AP (PPP2R5E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 23885-1-AP

    IHC staining of human cervical cancer using 23885-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23885-1-AP (PPP2R5E Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cervical cancer using 23885-1-AP

    IHC staining of human cervical cancer using 23885-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23885-1-AP (PPP2R5E Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 23885-1-AP

    IF Staining of HeLa using 23885-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23885-1-AP (PPP2R5E antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PPP2R5E Polyclonal antibody

PPP2R5E Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23885-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

PPP2R5E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23885-1-AP targets PPP2R5E in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原 PPP2R5E fusion protein Ag20891 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 2, regulatory subunit B', epsilon isoform
别名 PPP2R5E
计算分子量 467 aa, 55 kDa
观测分子量 51 kDa
GenBank蛋白编号BC092477
基因名称 PPP2R5E
Gene ID (NCBI) 5529
RRIDAB_2879350
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ16537
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP2R5E antibody 23885-1-APDownload protocol
IHC protocol for PPP2R5E antibody 23885-1-APDownload protocol
IF protocol for PPP2R5E antibody 23885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Protein Cell

Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice

Authors - He-Zhou Huang
View Article