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验证数据展示

  • IHC staining of mouse liver using 11276-1-AP

    IHC staining of mouse liver using 11276-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11276-1-AP (PPP2R5C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP2R5C antibody included
  • IHC staining of mouse liver using 11276-1-AP

    IHC staining of mouse liver using 11276-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11276-1-AP (PPP2R5C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP2R5C antibody included
  • WB analysis using 11276-1-AP

    WB analysis using 11276-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11276-1-AP (PPP2R5C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PPP2R5C Polyclonal antibody

PPP2R5C Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11276-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

B56G, KIAA0044, PP2A B subunit isoform B gamma, PPP2R5C, PR61G

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PPP2R5C Ready-To-Use IHC Kit. PPP2R5C primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11276-1-AP targets PPP2R5C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1819

Product name: Recombinant human PPP2R5C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-384 aa of BC016183

Sequence: MLTCNKAGSRMVVDAANSNGPFQPVVLLHIRDVPPADQEKLFIQKLRQCCVLFDFVSDPLSDLKWKEVKRAALSEMVEYITHNRNVITEPIYPEVVHMFAVNMFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNIAKKYIDQKFVLQLLELFDSEDPRERDFLKTTLHRIYGKFLGLRAYIRKQINNIFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKIFLLKVLLPLHKVKSLSVYHPQLAYCVVQFLEKDSTLTEPVVMALLKYWPKTHSPKEVMFLNELEEILDVIEPSEFVKIMEPLFRQLAKCVSSPHFQVAERALYYWNNEYIMSLISDNAAKILPIMFPSLYRNSKTHWNK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 2, regulatory subunit B', gamma isoform
别名 B56G, KIAA0044, PP2A B subunit isoform B gamma, PPP2R5C, PR61G
计算分子量 61 kDa
观测分子量 53-61 kDa
GenBank蛋白编号BC016183
基因名称 PPP2R5C
Gene ID (NCBI) 5527
RRIDAB_3669125
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13362
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP2R5C antibody 11276-1-APDownload protocol
IHC protocol for PPP2R5C antibody 11276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols