验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue, human brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| CoIP | See 1 publications below |
产品信息
12747-1-AP targets PPP2R2C in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PPP2R2C fusion protein Ag3470 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 2 (formerly 2A), regulatory subunit B, gamma isoform |
| 别名 | B55 GAMMA, IMYPNO, IMYPNO1, PP2A subunit B isoform gamma, PPP2R2C, PR52, PR55G |
| 计算分子量 | 430 aa, 49 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC032954 |
| 基因名称 | PPP2R2C |
| Gene ID (NCBI) | 5522 |
| RRID | AB_2170099 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2T4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP2R2C antibody 12747-1-AP | Download protocol |
| IHC protocol for PPP2R2C antibody 12747-1-AP | Download protocol |
| FC protocol for PPP2R2C antibody 12747-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Protein Cell Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice | ||
J Biochem Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell. | ||
Biochim Biophys Acta Mol Cell Res Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport | ||
Toxicol Appl Pharmacol Chronic arsenic exposure suppresses ATM pathway activation in human keratinocytes | ||
Cell Signal METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression |
