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验证数据展示

  • IHC staining of mouse brain using 13123-1-AP (same clone as 13123-1-PBS)

    IHC staining of mouse brain using 13123-1-AP (same clone as 13123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPP2R2B antibody included
  • WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

    WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

  • WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

    WB analysis using 13123-1-AP (same clone as 13123-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 13123-1-AP (same clone as 13123-1-PBS)

    IF Staining of SH-SY5Y using 13123-1-AP (same clone as 13123-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP2R2B/A/C/D antibody (13123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

  • IP experiment of SH-SY5Y using 13123-1-AP (same clone as 13123-1-PBS)

    IP experiment of SH-SY5Y using 13123-1-AP (same clone as 13123-1-PBS)

    IP result of anti-PPP2R2B/A/C/D (IP:13123-1-AP, 4ug; Detection:13123-1-AP 1:500) with SH-SY5Y cells lysate 1200ug. This data was developed using the same antibody clone with 13123-1-PBS in a different storage buffer formulation.

PPP2R2B/A/C/D Polyclonal antibody, PBS Only

PPP2R2B/A/C/D Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13123-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® PPP2R2B Ready-To-Use IHC Kit. PPP2R2B primary antibody included.

产品信息

13123-1-PBS targets PPP2R2B/A/C/D in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3860

Product name: Recombinant human PPP2R2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-443 aa of BC031790

Sequence: QQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEGRLRDPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQSFNIVDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFFSEIISSISDVKFSHSGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAWHPSENIIAVAATNNLYIFQDKVN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
别名
计算分子量 443 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC031790
基因名称 PPP2R2B
Gene ID (NCBI) 5521
RRIDAB_2169964
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ00005
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody has cross-reactivity to PPP2R2A, PPP2R2C and PPP2R2D.

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