IHCeasy® PPP2R2B Ready-To-Use IHC Kit
PPP2R2B Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat no : KHC3246
Synonyms
B55 BETA, B55β, PP2A B55BETA, PP2A PR55B, PP2A subunit B isoform B55-beta
Validation Data Gallery
Product Information
KHC3246 is a ready-to-use IHC kit for staining of PPP2R2B. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy® PPP2R2B Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
The PPP2R2B gene encodes a deduced 443-amino acid protein, which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme. PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. Primary antibody in this kit has cross-reactivity to PPP2R2A, PPP2R2C and PPP2R2D.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | B55 BETA, B55β, PP2A B55BETA, PP2A PR55B, PP2A subunit B isoform B55-beta |
