验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, U2OS cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | rat kidney tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| CoIP | See 2 publications below |
产品信息
12554-2-AP targets PPP2CB in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3233 Product name: Recombinant human PPP2CB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-309 aa of BC012022 Sequence: MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform |
| 别名 | Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, PP2CB, PP2A-beta, PP2A beta, EC:3.1.3.16 |
| 计算分子量 | 309 aa, 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC012022 |
| 基因名称 | PPP2CB |
| Gene ID (NCBI) | 5516 |
| RRID | AB_2169651 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62714 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPP2CB antibody 12554-2-AP | Download protocol |
| IP protocol for PPP2CB antibody 12554-2-AP | Download protocol |
| WB protocol for PPP2CB antibody 12554-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci GADD45α alleviates acetaminophen-induced hepatotoxicity by promoting AMPK activation.
| ||
Front Cell Dev Biol BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells. | ||
Cell Signal METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression |
