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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 67809-1-Ig (same clone as 67809-1-PBS)

    WB analysis using 67809-1-Ig (same clone as 67809-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67809-1-Ig (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67809-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 67809-1-Ig (same clone as 67809-1-PBS)

    IHC staining of human liver using 67809-1-Ig (same clone as 67809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67809-1-Ig (PPP2CA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67809-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPP2CA antibody included
  • FC experiment of MCF-7 using 67809-1-PBS

    FC experiment of MCF-7 using 67809-1-PBS

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PPP2CA Monoclonal antibody, PBS Only (67809-1-PBS, Clone:3A6H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

PPP2CA Monoclonal antibody, PBS Only

PPP2CA Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67809-1-PBS

产品说明书

CloneNo. 3A6H5

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

3A6H5, EC:3.1.3.16, PP2A alpha, PP2A-alpha, PP2Ac

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PPP2CA Ready-To-Use IHC Kit. PPP2CA primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67809-1-PBS targets PPP2CA in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag4294

Product name: Recombinant human PPP2CA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-309 aa of BC031696

Sequence: MDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
别名 3A6H5, EC:3.1.3.16, PP2A alpha, PP2A-alpha, PP2Ac
计算分子量 309 aa, 36 kDa
观测分子量33 kDa
GenBank蛋白编号BC031696
基因名称 PPP2CA
Gene ID (NCBI) 5515
RRIDAB_2918572
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP67775
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

实验方案

Product Specific Protocols
FC protocol for PPP2CA antibody 67809-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols