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PPP2CA Monoclonal antibody

PPP2CA Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A6H5

Cat No : 67809-1-Ig

Print datasheet

Synonyms

PP2A alpha, PP2Ac, PP2CA, PPP2CA, Replication protein C, RP C

验证数据展示

  • WB analysis using 67809-1-Ig

    WB analysis using 67809-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67809-1-Ig (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 67809-1-Ig

    IHC staining of human liver using 67809-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67809-1-Ig (PPP2CA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67809-1-Ig targets PPP2CA in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, rat
Cited Reactivityhuman
Immunogen PPP2CA fusion protein Ag4294 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Synonyms PP2A alpha, PP2Ac, PP2CA, PPP2CA, Replication protein C, RP C
Calculated Molecular Weight 309 aa, 36 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC031696
Gene Symbol PPP2CA
Gene ID (NCBI) 5515
RRIDAB_2918572
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP67775
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

实验方案

Product Specific Protocols
WB protocol for PPP2CA antibody 67809-1-IgDownload protocol
IHC protocol for PPP2CA antibody 67809-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma

Authors - Yue Li
View Article