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验证数据展示

  • WB analysis of PC-3 using 16115-1-AP

    WB analysis of PC-3 using 16115-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16115-1-AP (PPP1R8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16115-1-AP

    IHC staining of human kidney using 16115-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PPP1R8 antibody included
  • IHC staining of human kidney using 16115-1-AP

    IHC staining of human kidney using 16115-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PPP1R8 antibody included
  • IF Staining of HepG2 using 16115-1-AP

    IF Staining of HepG2 using 16115-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16115-1-AP (PPP1R8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PPP1R8 Polyclonal antibody

PPP1R8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16115-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

NIPP1, NIPP 1, ARD-1, ARD1, ARD 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PPP1R8 Ready-To-Use IHC Kit. PPP1R8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16115-1-AP targets PPP1R8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9041

Product name: Recombinant human PPP1R8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-209 aa of BC013360

Sequence: MGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 8
别名 NIPP1, NIPP 1, ARD-1, ARD1, ARD 1
计算分子量 209 aa, 23 kDa
观测分子量 47 kDa, 28 kDa, 19 kDa
GenBank蛋白编号BC013360
基因名称 PPP1R8
Gene ID (NCBI) 5511
RRIDAB_2169304
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12972
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R8, also named as ARD1, NIPP1, is a 351 amino acid protein, which has a basic N- and C-terminal and an acidic central domain. PPP1R8 is ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. PPP1R8 has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates and may also be involved in pre-mRNA splicing. PPP1R8 exists many isoforms and molecular weight of modified isoforms is about 18 kDa, 28 kDa and 47 kDa.

实验方案

Product Specific Protocols
IF protocol for PPP1R8 antibody 16115-1-APDownload protocol
IHC protocol for PPP1R8 antibody 16115-1-APDownload protocol
WB protocol for PPP1R8 antibody 16115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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