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CPI17 Polyclonal antibody

CPI17 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11658-1-AP

Print datasheet

Synonyms

CPI 17, CPI17, PPP1INL, PPP1R14A

验证数据展示

  • WB analysis of MCF-7 using 11658-1-AP

    WB analysis of MCF-7 using 11658-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 11658-1-AP

    IF Staining of PC-3 using 11658-1-AP

    Immunofluorescent analysis of PC-3 cells using 11658-1-AP (CPI17 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells, human colon tissue, Jurkat cells, PC-3 cells
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11658-1-AP targets CPI17 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen CPI17 fusion protein Ag2237 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 14A
Synonyms CPI 17, CPI17, PPP1INL, PPP1R14A
Calculated Molecular Weight 147 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC021089
Gene Symbol PPP1R14A
Gene ID (NCBI) 94274
RRIDAB_2252794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96A00
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R14A, also named as CPI17, belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. CPI17 has two isoforms:CPI-17alpha (17 kDa) and CPI-17beta (13 kDa).

实验方案

Product Specific Protocols
WB protocol for CPI17 antibody 11658-1-APDownload protocol
IF protocol for CPI17 antibody 11658-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols