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  • Featured Product
  • KD/KO Validated

PPP1CB Polyclonal antibody

PPP1CB Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 55136-1-AP

Print datasheet

Synonyms

PP1 Beta, PP 1B, PPP1CB, PPP1CD

验证数据展示

  • WB analysis using 55136-1-AP

    WB analysis using 55136-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55136-1-AP (PPP1CB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 55136-1-AP

    IF Staining of A549 using 55136-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 55136-1-AP (PPP1CB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, BxPC-3 cells, NIH/3T3 cells, SH-SY5Y cells, mouse brain tissue
Positive IF detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CB Ready-To-Use IHC Kit. PPP1CB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55136-1-AP targets PPP1CB in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, catalytic subunit, beta isoform
Synonyms PP1 Beta, PP 1B, PPP1CB, PPP1CD
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37-40 kDa, 33 kDa
GenBank Accession NumberNM_002709
Gene Symbol PPP1CB
Gene ID (NCBI) 5500
RRIDAB_10837232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62140
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.

实验方案

Product Specific Protocols
WB protocol for PPP1CB antibody 55136-1-APDownload protocol
IF protocol for PPP1CB antibody 55136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Pathog

Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids.

Authors - Zhanying Hu
  • KD Validated
View Article
humanWB

Exp Eye Res

PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy.

Authors - Yang Guo
View Article