PDP1 Polyclonal antibody
PDP1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, mouse skeletal muscle tissue, mouse brain tissue, mouse heart tissue, mouse liver tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human prostate cancer tissue, human thyroid cancer tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PDP1 Ready-To-Use IHC Kit. PDP1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
21176-1-AP targets PDP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | PDP1 fusion protein Ag15453 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | protein phosphatase 2C, magnesium-dependent, catalytic subunit |
| Synonyms | PDH, PDP, PDP 1, PDP1, PDPC, PDPC 1, PPM2C, Protein phosphatase 2C |
| Calculated Molecular Weight | 537 aa, 61 kDa |
| Observed Molecular Weight | 53-61 kDa |
| GenBank Accession Number | BC047619 |
| Gene Symbol | PPM2C |
| Gene ID (NCBI) | 54704 |
| RRID | AB_2878824 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P0J1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDP1 antibody 21176-1-AP | Download protocol |
| IHC protocol for PDP1 antibody 21176-1-AP | Download protocol |
| IP protocol for PDP1 antibody 21176-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Identification of immune-related cells and genes in the breast invasive carcinoma microenvironment. | ||
Curr Med Sci Salidroside Inhibits Lipopolysaccharide-ethanol-induced Activation of Proinflammatory Macrophages via Notch Signaling Pathway. | ||
J Diabetes Res Pyruvate-Enriched Oral Rehydration Solution Improves Glucometabolic Disorders in the Kidneys of Diabetic db/db Mice. | ||
Cell Death Dis Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca2+]m-PDP1-PDH-histone acetylation retrograde signaling. | ||
ACS Chem Neurosci FDCA Attenuates Neuroinflammation and Brain Injury after Cerebral Ischemic Stroke |
