验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
18203-1-AP targets PPM1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag12775 Product name: Recombinant human PPM1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-233 aa of BC104885 Sequence: MPAFSTTAAEYVKSRLPEALKQHLQDYEKDKENSVLSYQTILEQQILSIDREMLEKLTVSYDEAGTTCLIALLSDKDLTVANVGDSRGVLCDKDGNAIPLSHDHKPYQLKERKRIKRAGGFISFNGSWRVQGILAMSRSLGDYPLKNLNVVIPDPDILTFDLDKLQPEFMILASDGLWDAFSNEEAVRFIKERLDEPHFGAKSIVLQSFYRGCPDNITVMVVKFRNSSKTEEQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1 (formerly 2C)-like |
| 别名 | PP2C epsilon, PP2CE, PPM1 LIKE, PPM1L, Protein phosphatase 1 like, Protein phosphatase 1L |
| 计算分子量 | 26 kDa, 41 kDa |
| 观测分子量 | 41-45 kDa |
| GenBank蛋白编号 | BC104885 |
| 基因名称 | PPM1L |
| Gene ID (NCBI) | 151742 |
| RRID | AB_10859821 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5SGD2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPM1L(Protein phosphatase 1L) is also named as PP2CE.It belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. It has 4 isoforms produced by alternative splicing.This antibody is specific to PPM1L.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPM1L antibody 18203-1-AP | Download protocol |
| WB protocol for PPM1L antibody 18203-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
