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PPM1L Polyclonal antibody

PPM1L Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 18203-1-AP

Print datasheet

Synonyms

PP2C epsilon, PP2CE, PPM1 LIKE, PPM1L, Protein phosphatase 1 like, Protein phosphatase 1L

验证数据展示

  • WB analysis of mouse kidney using 18203-1-AP

    WB analysis of mouse kidney using 18203-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18203-1-AP (PPM1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 18203-1-AP

    IHC staining of human kidney using 18203-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18203-1-AP (PPM1L Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 18203-1-AP

    IHC staining of human kidney using 18203-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18203-1-AP (PPM1L Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 18203-1-AP is PPM1L Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PPM1L fusion protein Ag12775 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1 (formerly 2C)-like
Synonyms PP2C epsilon, PP2CE, PPM1 LIKE, PPM1L, Protein phosphatase 1 like, Protein phosphatase 1L
Calculated Molecular Weight 26 kDa, 41 kDa
Observed Molecular Weight 41-45 kDa
GenBank Accession NumberBC104885
Gene Symbol PPM1L
Gene ID (NCBI) 151742
RRIDAB_10859821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SGD2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPM1L(Protein phosphatase 1L) is also named as PP2CE.It belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. It has 4 isoforms produced by alternative splicing.This antibody is specific to PPM1L.

实验方案

Product Specific Protocols
WB protocol for PPM1L antibody 18203-1-APDownload protocol
IHC protocol for PPM1L antibody 18203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

Authors - Xiaoman Zhu
View Article