验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87805-1-RR targets PPM1K in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag41706 Product name: Recombinant human PPM1K protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-372 aa of BC037552 Sequence: MSTAALITLVRSGGNQVRRRVLLSSRLLQDDRRVTPTCHSSTSEPRCSRFDPDGSGSPATWDNFGIWDNRIDEPILLPPSIKYGKPIPKISLEKVGCASQIGKRKENEDRFDFAQLTDEVLYFAVYDGHGGPAAADFCHTHMEKCIMDLLPKEKNLETLLTLAFLEIDKAFSSHARLSADATLLTSGTTATVALLRDGIELVVASVGDSRAILCRKGKPMKLTIDHTPERKDEKERIKKCGGFVAWNSLGQPHVNGRLAMTRSIGDLDLKTSGVIAEPETKRIKLHHADDSFLVLTTDGINFMVNSQEICDFVNQCHDPNEAAHAVTEQAIQYGTEDNSTAVVVPFGAWGKYKNSEINFSFSRSFASSGRWA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1K (PP2C domain containing) |
| 别名 | [3-methyl-2-oxobutanoate dehydrogenase, 2-methylpropanoyl-transferring, BCKDH, BDP, Branched-chain alpha-ketoacid dehydrogenase phosphatase |
| 计算分子量 | 41 kDa |
| 观测分子量 | 37-41 kDa |
| GenBank蛋白编号 | BC037552 |
| 基因名称 | PPM1K |
| Gene ID (NCBI) | 152926 |
| RRID | AB_3745760 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8N3J5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein phosphatase, Mg2+/Mn2+ dependent 1K (PPM1K), is a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. PPMiK, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore.PPM1K is essential for the regulation and nutrient-induced activation of the branched-chain alpha-keto acid dehydrogenase complex (BCKD or BCKDC), which catalyzes the breakdown of branched-chain amino acids (BCAA).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPM1K antibody 87805-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
