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验证数据展示

  • WB analysis using 87250-3-RR (same clone as 87250-3-PBS)

    WB analysis using 87250-3-RR (same clone as 87250-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87250-3-RR (PPM1G antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87250-3-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 87250-3-RR (same clone as 87250-3-PBS)

    IF Staining of MCF-7 using 87250-3-RR (same clone as 87250-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (87250-3-RR, Clone: 252477B6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87250-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 87250-3-RR (same clone as 87250-3-PBS)

    IHC staining of mouse testis using 87250-3-RR (same clone as 87250-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 87250-3-RR (PPM1G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87250-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPM1G antibody included
  • Affinity of 87250-3-RR

    Affinity of 87250-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87250-3-RR against Human PPM1G were performed. The affinity constant is 4.11 nM.

PPM1G Recombinant monoclonal antibody, PBS Only

PPM1G Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87250-3-PBS

产品说明书

CloneNo. 252477B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:3.1.3.16, PP2C gamma, PP2CG, PP2CGAMMA, PP2C-gamma

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PPM1G Ready-To-Use IHC Kit. PPM1G primary antibody included.

产品信息

87250-3-PBS targets PPM1G in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7751

Product name: Recombinant human PPM1G protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 191-546 aa of BC000057

Sequence: GPEDSTRETPSQENGPTAKAYTGFSSNSERGTEAGQVGEPGIPTGEAGPSCSSASDKLPRVAKSKFFEDSEDESDEAEEEEEDSEECSEEEDGYSSEEAENEEDEDDTEEAEEDDEEEEEEMMVPGMEGKEEPGSDSGTTAVVALIRGKQLIVANAGDSRCVVSEAGKALDMSYDHKPEDEVELARIKNAGGKVTMDGRVNGGLNLSRAIGDHFYKRNKNLPPEEQMISALPDIKVLTLTDDHEFMVIACDGIWNVMSSQEVVDFIQSKISQRDENGELRLLSSIVEELLDQCLAPDTSGDGTGCDNMTCIIICFKPRNTAELQPESGKRKLEEVLSTEGAEENGNSDKKKKAKRD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
别名 EC:3.1.3.16, PP2C gamma, PP2CG, PP2CGAMMA, PP2C-gamma
计算分子量 59 kDa
观测分子量 70 kDa
GenBank蛋白编号BC000057
基因名称 PPM1G
Gene ID (NCBI) 5496
RRIDAB_3745431
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15355
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).

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