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验证数据展示

  • WB analysis using 87405-1-RR

    WB analysis using 87405-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87405-1-RR (PPM1E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 87405-1-RR

    IF Staining of mouse brain using 87405-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PPM1E antibody (87405-1-RR, Clone: 252801H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 87405-1-RR

    IF Staining of mouse brain using 87405-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PPM1E antibody (87405-1-RR, Clone: 252801H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPM1E Recombinant monoclonal antibody

PPM1E Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 87405-1-RR

产品说明书

CloneNo. 252801H3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

Ca(2+)/calmodulin-dependent protein kinase phosphatase N, CaMKN, CaMKP N, CaMKP-N, CaMKP-nucleus

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue, rat brain tissue, fetal human brain tissue
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87405-1-RR targets PPM1E in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18903

Product name: Recombinant human PPM1E protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 501-754 aa of BC136290

Sequence: EESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYDGRVDSFTDRTSLSPGSQINVLEDPGYLDLTQIEASKPHSAQFLLPVEMFGPGAPKKANLINELMMEKKSVQSSLPEWSGAGEFPTAFNLGSTGEQIYRMQSLSPVCSGLENEQFKSPGNRVSRLSHLRHHYSKKWHRFRFNPKFYSFLSAQEPSHKIGTSLSSLTGSGKRNRIRSSLPWRQNSWKGYSENMRKLRKTHDIPCPDLPWSY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein phosphatase 1E (PP2C domain containing)
别名 Ca(2+)/calmodulin-dependent protein kinase phosphatase N, CaMKN, CaMKP N, CaMKP-N, CaMKP-nucleus
计算分子量 764 aa, 85 kDa
观测分子量85 kDa
GenBank蛋白编号BC136290
基因名称 PPM1E
Gene ID (NCBI) 22843
RRIDAB_3745521
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8WY54
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N),also known as PPM1E, is an AMPKα phosphatase,which is up-regulated in human gastric cancer tissues (PMID: 28423719).

实验方案

Product Specific Protocols
IF protocol for PPM1E antibody 87405-1-RRDownload protocol
WB protocol for PPM1E antibody 87405-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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