验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PPM1D Ready-To-Use IHC Kit. PPM1D primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67440-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67440 targets PPM1D in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag24761 Product name: Recombinant human PPM1D protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 254-423 aa of BC060877 Sequence: NGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGEFVVSPEPDTSVHTLDPQKHKYIILGSDGLWNMIPQQDAISMCQDQEEKKYLMGEHGQSCAKMLVNRALGRWRQRMLRADNTSAIVICISPEVDNQGNFTNEDELYLNLTDSPSYNSQETCVMTPSPCSTPPVKSLE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1D magnesium-dependent, delta isoform |
| 别名 | PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1 |
| 观测分子量 | 67 kDa, 47 kDa |
| GenBank蛋白编号 | BC060877 |
| 基因名称 | PPM1D |
| Gene ID (NCBI) | 8493 |
| RRID | AB_2920136 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O15297 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 PPM1D antibody CL594-67440 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
