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验证数据展示

  • WB analysis using 14331-1-AP (same clone as 14331-1-PBS)

    WB analysis using 14331-1-AP (same clone as 14331-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14331-1-AP (PPIP5K1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14331-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 14331-1-AP (same clone as 14331-1-PBS)

    IHC staining of mouse skeletal muscle using 14331-1-AP (same clone as 14331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14331-1-AP (PPIP5K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14331-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 14331-1-AP (same clone as 14331-1-PBS)

    IF Staining of U-251 using 14331-1-AP (same clone as 14331-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PPIP5K1 antibody (14331-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14331-1-PBS in a different storage buffer formulation.

PPIP5K1 Polyclonal antibody, PBS Only

PPIP5K1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14331-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14331-1-PBS targets PPIP5K1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5614

Product name: Recombinant human HISPPD2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC050263

Sequence: MWSLTASEGESTTAHFFLGAGDEGLGTRGIGMRPEESDSELLEDEEDEVPPEPQIIVGICAMTKKSKSKPMTQILERLCRFDYLTVVILGEDVILNEPVENWPSCHCLISFHSKGFPLDKAVAYSKLRNPFLINDLAMQYYIQDRREVYRILQEEGIDLPRYAVLNRDPARPEECNLIEGEDQVEVNGAVFPKPFVEKPVSAEDHNVYIYYPSSAGGGSQRLFRKIGSRSSVYSPESSVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEIRYPVMLTAME

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histidine acid phosphatase domain containing 2A
别名
计算分子量 160 kDa
观测分子量160 kDa
GenBank蛋白编号BC050263
基因名称 HISPPD2A
Gene ID (NCBI) 9677
RRIDAB_3669185
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PFW1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPIP5K1 (Inositol diphosphoinositol pentakisphosphate kinase type 1) plays an important role in regulating cell death in response to genotoxic stress. PPIP5K1 act in concert with IP6K2 to promote cell death through controlling localization of stress-activated MAP kinase-interacting protein1 (SIN1) (PMID: 31051014). The activation of the PtdIns 3-kinase pathway is associated with PPIP5K1 translocation from the cytoplasm to the plasma membrane (PMID: 21222653).

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