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验证数据展示

  • WB analysis using 14331-1-AP

    WB analysis using 14331-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14331-1-AP (PPIP5K1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 14331-1-AP

    IHC staining of mouse skeletal muscle using 14331-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14331-1-AP (PPIP5K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 14331-1-AP

    IF Staining of U-251 using 14331-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PPIP5K1 antibody (14331-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PPIP5K1 Polyclonal antibody

PPIP5K1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14331-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

HISPPD2A, EC:2.7.4.24, InsP6 and PP-IP5 kinase 1, Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1, hsVIP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14331-1-AP targets PPIP5K1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PPIP5K1 fusion protein Ag5614 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histidine acid phosphatase domain containing 2A
别名 HISPPD2A, EC:2.7.4.24, InsP6 and PP-IP5 kinase 1, Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1, hsVIP1
计算分子量 160 kDa
观测分子量160 kDa
GenBank蛋白编号BC050263
基因名称 HISPPD2A
Gene ID (NCBI) 9677
RRIDAB_3669185
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PFW1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPIP5K1 (Inositol diphosphoinositol pentakisphosphate kinase type 1) plays an important role in regulating cell death in response to genotoxic stress. PPIP5K1 act in concert with IP6K2 to promote cell death through controlling localization of stress-activated MAP kinase-interacting protein1 (SIN1) (PMID: 31051014). The activation of the PtdIns 3-kinase pathway is associated with PPIP5K1 translocation from the cytoplasm to the plasma membrane (PMID: 21222653).

实验方案

Product Specific Protocols
WB protocol for PPIP5K1 antibody 14331-1-APDownload protocol
IHC protocol for PPIP5K1 antibody 14331-1-APDownload protocol
IF protocol for PPIP5K1 antibody 14331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols