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PPIL6 Polyclonal antibody

PPIL6 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17452-1-AP

Print datasheet

Synonyms

Cyclophilin like protein PPIL6, PPIase, PPIL6, Rotamase PPIL6

验证数据展示

  • WB analysis of HeLa using 17452-1-AP

    WB analysis of HeLa using 17452-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17452-1-AP (PPIL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17452-1-AP

    IF Staining of HeLa using 17452-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17452-1-AP (PPIL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

17452-1-AP targets PPIL6 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PPIL6 fusion protein Ag11578 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidylprolyl isomerase (cyclophilin)-like 6
Synonyms Cyclophilin like protein PPIL6, PPIase, PPIL6, Rotamase PPIL6
Calculated Molecular Weight 311 aa, 35 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC038716
Gene Symbol PPIL6
Gene ID (NCBI) 285755
RRIDAB_2237569
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPIL6 antibody 17452-1-APDownload protocol
IF protocol for PPIL6 antibody 17452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Curr Biol

CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement

Authors - Chao Liu
View Article
mouseWB

Cell Mol Life Sci

CCDC146 is required for sperm flagellum biogenesis and male fertility in mice

Authors - Yanjie Ma
View Article