验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A375 cells, K-562 cells, human heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86312-1-RR targets PPIL1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag7436 Product name: Recombinant human PPIL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC003048 Sequence: MAAIPPDSWQPPNVYLETSMGIIVLELYWKHAPKTCKNFAELARRGYYNGTKFHRIIKDFMIQGGDPTGTGRGGASIYGKQFEDELHPDLKFTGAGILAMANAGPDTNGSQFFVTLAPTQWLDGKHTIFGRVCQGIGMVNRVGMVETNSQDRPVDDVKIIKAYPSG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | peptidylprolyl isomerase (cyclophilin)-like 1 |
| 别名 | CGI 124, CGI-124, CYPL1, EC:5.2.1.8, hCyPX |
| 计算分子量 | 18 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC003048 |
| 基因名称 | PPIL1 |
| Gene ID (NCBI) | 51645 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9Y3C6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Peptidyl-prolyl isomerase (PPIase) catalyzes the interconversion of a specific Pro-imide bond between the cis and trans conformations. PPIL1 (Peptidyl-prolyl cis-trans isomerase-like 1) is up-regulated in human colon cancer cells and an siRNA-mediated knockdown of PPIL1 resulted in a reduced number of viable cell (PMID: 20368803). Expression of PPIL1 was shown to be ubiquitous across all adult tissues (with highest expression in the adrenal gland, testis and heart). PPIL1 is also known to participate in the spliceosome; a complex and dynamic protein and RNA complex responsible for splicing introns from pre-mRNA (PMID: 33220177, 8978786).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPIL1 antibody 86312-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
