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验证数据展示

  • WB analysis using 15357-1-AP

    WB analysis using 15357-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15357-1-AP (Cyclophilin E antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15357-1-AP

    IF Staining of HeLa using 15357-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclophilin E antibody (15357-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Cyclophilin E Polyclonal antibody

Cyclophilin E Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15357-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

PPIE, Cyclophilin 33, Cyclophilin-33, CYP 33, CYP33

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15357-1-AP targets Cyclophilin E in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7603

Product name: Recombinant human PPIE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC004898

Sequence: MATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAAAAIDNMNESELFGRTIRVNLAKPMRIKEGSSRPVWSDDDWLKKFSGKTLEENKEEEGSEPPKAETQEGEPIAKKARSNPQVYMDIKIGNKPAGRIQMLLRSDVVPMTAENFRCLCTHEKGFGFKGSSFHRIIPQFMCQGGDFTNHNGTGGKSIYGKKFDDENFILKHTGPGLLSMANSGPNTNGSQFFLTCDKTDWLDGKHVVFGEVTEGLDVLRQIEAQGSKDGKPKQKVIIADCGEYV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidylprolyl isomerase E (cyclophilin E)
别名 PPIE, Cyclophilin 33, Cyclophilin-33, CYP 33, CYP33
计算分子量 33 kDa
观测分子量 33-38 kDa
GenBank蛋白编号BC004898
基因名称 Cyclophilin E
Gene ID (NCBI) 10450
RRIDAB_2878131
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNP9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclophilin E (CypE), also known as Cyclophilin-33, Rotamase E, CYP33, PPIE, is an enzyme which belongs to the cyclophilin-type PPIase family of PPIase E subfamily (PMID: 15998457). Cyclophilin E negatively regulates influenza virus replication and transcription occurs by impairing the formation of the vRNP (PMID: 21887220). Additionally, Cyclophilin E plays an important role in osteoblast differentiation as a positive regulator by increasing the transcriptional activity of Runx2 (PMID: 37947627).

实验方案

Product Specific Protocols
WB protocol for Cyclophilin E antibody 15357-1-APDownload protocol
IF protocol for Cyclophilin E antibody 15357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols