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验证数据展示

  • WB analysis of Jurkat using 51040-1-AP

    WB analysis of Jurkat using 51040-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 51040-1-AP (Cyclophilin A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Cyclophilin A Polyclonal antibody

Cyclophilin A Polyclonal Antibody for WB, ELISA
Cat No. 51040-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Cyclophilin A, Cyclosporin A binding protein, CYPA, CYPH, PPIA, PPIase A, Rotamase A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy PPIA Ready-To-Use IHC Kit. PPIA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

51040-1-AP targets Cyclophilin A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag0480

Product name: Recombinant human PPIA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-165 aa of BC000689

Sequence: MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidylprolyl isomerase A (cyclophilin A)
别名 Cyclophilin A, Cyclosporin A binding protein, CYPA, CYPH, PPIA, PPIase A, Rotamase A
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC000689
基因名称 PPIA
Gene ID (NCBI) 5478
RRIDAB_2237515
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62937
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPIA, also named as CYPA and Rotamase A, belongs to the cyclophilin-type PPIase family and PPIase A subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIA forms a trimolecular complex with cyclophilin and calcineurins to inhibit calcineurin phosphatase activity. PPIA is incorporated into the virion of the type 1 human immunodeficiency virus (HIV-1) via a direct interaction with the capsid domain of the viral Gag polyprotein and is crucial for efficient viral replication.

实验方案

Product Specific Protocols
WB protocol for Cyclophilin A antibody 51040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Oncol

Proteomics-based identification of a group of apoptosis-related proteins and biomarkers in gastric cancer.

Authors - Zhigang Bai
View Article