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PPFIBP1 Polyclonal antibody

PPFIBP1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 13961-1-AP

Print datasheet

Synonyms

hSGT2, hSgt2p, KIAA1230, L2, Liprin beta 1, PPFIBP1

验证数据展示

  • WB analysis of HepG2 using 13961-1-AP

    WB analysis of HepG2 using 13961-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13961-1-AP (PPFIBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 13961-1-AP

    IHC staining of human colon cancer using 13961-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 13961-1-AP (PPFIBP1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon cancer using 13961-1-AP

    IHC staining of human colon cancer using 13961-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 13961-1-AP (PPFIBP1 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 13961-1-AP

    IP experiment of HepG2 using 13961-1-AP

    IP result of anti-PPFIBP1 (IP:13961-1-AP, 4ug; Detection:13961-1-AP 1:500) with HepG2 cells lysate 2400ug.

  • IF Staining of HepG2 using 13961-1-AP

    IF Staining of HepG2 using 13961-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13961-1-AP (PPFIBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 13961-1-AP

    FC experiment of HepG2 using 13961-1-AP

    1X10^6 HepG2 cells were stained with .2ug PPFIBP1 antibody (13961-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

经过测试的应用

Positive WB detected inHepG2 cells, DU 145 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13961-1-AP targets PPFIBP1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PPFIBP1 fusion protein Ag4884 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PTPRF interacting protein, binding protein 1 (liprin beta 1)
Synonyms hSGT2, hSgt2p, KIAA1230, L2, Liprin beta 1, PPFIBP1
Calculated Molecular Weight 1011 aa, 114 kDa
Observed Molecular Weight 114 kDa
GenBank Accession NumberBC050281
Gene Symbol PPFIBP1
Gene ID (NCBI) 8496
RRIDAB_2171631
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86W92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPFIBP1 antibody 13961-1-APDownload protocol
IHC protocol for PPFIBP1 antibody 13961-1-APDownload protocol
IF protocol for PPFIBP1 antibody 13961-1-APDownload protocol
IP protocol for PPFIBP1 antibody 13961-1-APDownload protocol
FC protocol for PPFIBP1 antibody 13961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Open Biol

The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesions

Authors - Xingchen Li
View Article