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验证数据展示

  • WB analysis using 83142-4-RR (same clone as 83142-4-PBS)

    WB analysis using 83142-4-RR (same clone as 83142-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83142-4-RR (PPFIA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83142-4-RR (same clone as 83142-4-PBS)

    FC experiment of HEK-293 using 83142-4-RR (same clone as 83142-4-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83142-4-RR (same clone as 83142-4-PBS)

    FC experiment of U-251 using 83142-4-RR (same clone as 83142-4-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 83142-4-RR (same clone as 83142-4-PBS)

    WB analysis of HEK-293 using 83142-4-RR (same clone as 83142-4-PBS)

    WB result of PPFIA3 antibody (83142-4-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPFIA3 transfected HEK-293 cells. This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 83142-4-RR (same clone as 83142-4-PBS)

    IP experiment of mouse brain using 83142-4-RR (same clone as 83142-4-PBS)

    IP result of anti-PPFIA3 (IP:83142-4-RR, 4ug; Detection:83142-4-RR 1:4000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 83142-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00170-2

    Cytometric bead array standard curve of MP00170-2

    Cytometric bead array standard curve of MP00170-2, PPFIA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83142-4-PBS. Detection antibody: 83142-3-PBS. Standard: Ag1971. Range: 1.25-20 ng/mL.

  • Cytometric bead array standard curve of MP00170-3

    Cytometric bead array standard curve of MP00170-3

    Cytometric bead array standard curve of MP00170-3, PPFIA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83142-4-PBS. Detection antibody: 83142-2-PBS. Standard: Ag1971. Range: 1.25-20 ng/mL.

  • Affinity and Kinetic Characterization of 83142-4-RR

    Affinity and Kinetic Characterization of 83142-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83142-4-RR against Human PPFIA3 were performed. The affinity constant is 0.131 nM.

  • Featured Product
  • KD/KO验证
    • {{ptg:ProductFeaturesInfo}}

PPFIA3 Recombinant monoclonal antibody, PBS Only (Capture)

PPFIA3 Uni-rAb® Recombinant Antibody for WB, FC (Intra), IP, Cytometric bead array, Indirect ELISA
Cat No. 83142-4-PBS

产品说明书

CloneNo. 230459F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), IP, Cytometric bead array, Indirect ELISA

别名

230459F6, KIAA0654, Liprin-alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, PTPRF-interacting protein alpha-3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PPFIA3 Ready-To-Use IHC Kit. PPFIA3 primary antibody included.

产品信息

83142-4-PBS targets PPFIA3 as part of a matched antibody pair:

MP00170-2: 83142-4-PBS capture and 83142-3-PBS detection (validated in Cytometric bead array)

MP00170-3: 83142-4-PBS capture and 83142-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1971

Product name: Recombinant human PPFIA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-277 aa of BC021255

Sequence: APASSRTSTGNVWMTHEEMESLTATTKPILAYGDMNHEWVGNDWLPSLGLPQYRSYFMESLVDARMLDHLNKKELRGQLKMVDSFHRVSLHYGIMCLKRLNYDRKDLERRREESQTQIRDVMVWSNERVMGWVSGLGLKEFATNLTESGVHGALLALDETFDYSDLALLLQIPTQNAQARQLLEKEFSNLISLGTDRRLDEDSAKSFSRSPSWRKMFREKDLRGVTPDSAEMLPPNFRSAAAGALGSPGLPLRKLQPEGQTSGSSRADGVSVRTYSC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
别名 230459F6, KIAA0654, Liprin-alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, PTPRF-interacting protein alpha-3
计算分子量 1194 aa, 133 kDa
观测分子量 140 kDa
GenBank蛋白编号BC021255
基因名称 PPFIA3
Gene ID (NCBI) 8541
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75145
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPFIA3, also named as KIAA0654, belongs to the liprin family and Liprin-alpha subfamily. PPFIA3 may regulate the disassembly of focal adhesions. It may localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates. PPFIA3 exhibites more than 5 fold accumulations in the monoribosome fraction. PPFIA3 is decreased at the protein level by the miR-99 family but not in the luciferase reporter with the 3'UTR of PPFIA3. (PMID:21212412)