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验证数据展示

  • IHC staining of mouse ovary using 20203-1-AP

    IHC staining of mouse ovary using 20203-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 20203-1-AP (POTEA-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary using 20203-1-AP

    IHC staining of human ovary using 20203-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20203-1-AP (POTEA-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 20203-1-AP

    IF Staining of HepG2 using 20203-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using POTEA-Specific antibody (20203-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

POTEA-Specific Polyclonal antibody

POTEA-Specific Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 20203-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

A26A1, ANKRD26 like family A member 1, POTE 8, POTE8, POTEA, POTEA-Specific

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse ovary tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20203-1-AP targets POTEA-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 POTE ankyrin domain family, member A
别名 A26A1, ANKRD26 like family A member 1, POTE 8, POTE8, POTEA, POTEA-Specific
计算分子量 56 kDa
GenBank蛋白编号NM_001002920
基因名称 POTEA
Gene ID (NCBI) 340441
RRIDAB_10666735
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6S8J7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POTEA, also named as A26A1 and POTE8, belongs to the POTE family. POTE has an important signaling function in the reproductive system. The antibody is specific to POTEA.

实验方案

Product Specific Protocols
IHC protocol for POTEA-Specific antibody 20203-1-APDownload protocol
IF protocol for POTEA-Specific antibody 20203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols