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验证数据展示

  • WB analysis using 24701-1-AP (same clone as 24701-1-PBS)

    WB analysis using 24701-1-AP (same clone as 24701-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24701-1-AP (POLR3G antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24701-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 24701-1-AP (same clone as 24701-1-PBS)

    IP experiment of HeLa using 24701-1-AP (same clone as 24701-1-PBS)

    IP result of anti-POLR3G (IP:24701-1-AP, 4ug; Detection:24701-1-AP 1:3000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 24701-1-PBS in a different storage buffer formulation.

POLR3G Polyclonal antibody, PBS Only

POLR3G Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 24701-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

RPC7, RPC32-alpha, RPC32, RNA polymerase III 32 kDa apha subunit, DNA-directed RNA polymerase III subunit RPC7

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® POLR3G Ready-To-Use IHC Kit. POLR3G primary antibody included.

产品信息

24701-1-PBS targets POLR3G in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19872

Product name: Recombinant human POLR3G protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-223 aa of BC141649

Sequence: MAGNKGRGRAAYTFNIEAVGFSKGEKLPDVVLKPPPLFPDTDYKPVPLKTGEGEEYMLALKQELRETMKRMPYFIETPEERQDIERYSKRYMKVYKEEWIPDWRRLPREMMPRNKCKKAGPKPKKAKDAGKGTPLTNTEDVLKKMEELEKRGDGEKSDEENEEKEGSKEKSKEGDDDDDDDAAEQEEYDEEEQEEENDYINSYFEDGDDFGADSDDNMDEATY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (RNA) III (DNA directed) polypeptide G (32kD)
别名 RPC7, RPC32-alpha, RPC32, RNA polymerase III 32 kDa apha subunit, DNA-directed RNA polymerase III subunit RPC7
计算分子量 223 aa, 26 kDa
观测分子量 26-30 kDa
GenBank蛋白编号BC141649
基因名称 POLR3G
Gene ID (NCBI) 10622
RRIDAB_2879681
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15318
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POLR3G also named as RNA polymerase III subunit C7 is a 223 amino acid protein, which belongs to eukaryotic RPC7 RNA polymerase subunit family. The molecular weight of POLR3G is 26 kDa. POLR3G localizes in the nucleus and is a component of the RNA polymerase III complex, which may be involved either in the recruitment and stablization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation.

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