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Host
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0
Conjugate
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验证数据展示

  • WB analysis of COS-7 using 84357-1-RR (same clone as 84357-1-PBS)

    WB analysis of COS-7 using 84357-1-RR (same clone as 84357-1-PBS)

    COS-7 cells were subjected to SDS PAGE followed by western blot with 84357-1-RR (POLR2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 84357-1-RR (same clone as 84357-1-PBS)

    IHC staining of mouse pancreas using 84357-1-RR (same clone as 84357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84357-1-RR (POLR2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84357-1-RR (same clone as 84357-1-PBS)

    IF Staining of HepG2 using 84357-1-RR (same clone as 84357-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2A antibody (84357-1-RR, Clone: 240869E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

POLR2A Recombinant antibody, PBS Only

POLR2A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84357-1-PBS

产品说明书

CloneNo. 240869E9

宿主/亚型

Rabbit / IgG

种属反应性

human, monkey

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A, 3'-5' exoribonuclease

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84357-1-PBS targets POLR2A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, monkey samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, monkey
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
别名 EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A, 3'-5' exoribonuclease
计算分子量 217 kDa
观测分子量245 kDa
GenBank蛋白编号NM_000937
基因名称 POLR2A
Gene ID (NCBI) 5430
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP24928
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.