验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, NIH/3T3 cells |
| Positive IHC detected in | human pancreas cancer tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20655-1-AP targets POLR2A in WB, IHC, IF, ELISA applications and shows reactivity with human, monkey, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, monkey, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polymerase (RNA) II (DNA directed) polypeptide A, 220kDa |
| 别名 | hRPB220, hsRPB1, Pol II, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2 |
| 计算分子量 | 217 kDa |
| 观测分子量 | 210-250 kDa |
| GenBank蛋白编号 | NM_000937 |
| 基因名称 | POLR2A |
| Gene ID (NCBI) | 5430 |
| RRID | AB_2878716 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P24928 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for POLR2A antibody 20655-1-AP | Download protocol |
| WB protocol for POLR2A antibody 20655-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Brahma-Related Gene 1 Deficiency in Endothelial Cells Ameliorates Vascular Inflammatory Responses in Mice. | ||
Cell Death Discov Cezanne promoted autophagy through PIK3C3 stabilization and PIK3C2A transcription in lung adenocarcinoma
| ||
Cell Rep NEIL1 drives the initiation of colorectal cancer through transcriptional regulation of COL17A1 | ||
Nat Commun Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1 | ||
Neuropharmacology Neurotransmitter Imbalance and Amygdala Synaptic Plasticity in Lumbar Disc Herniation-induced Chronic Pain and Related Emotional Disturbances:A multi-omics analysis |
