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POLR2A Polyclonal antibody

POLR2A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey, mouse

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20655-1-AP

Print datasheet

Synonyms

hRPB220, hsRPB1, Pol II, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2

验证数据展示

  • WB analysis of HeLa using 20655-1-AP

    WB analysis of HeLa using 20655-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 20655-1-AP

    WB analysis using 20655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 20655-1-AP

    IHC staining of human pancreas cancer using 20655-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20655-1-AP (POLR2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 20655-1-AP

    IHC staining of mouse pancreas using 20655-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20655-1-AP (POLR2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20655-1-AP targets POLR2A in WB, IF, IHC, ELISA applications and shows reactivity with human, monkey, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, monkey, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Synonyms hRPB220, hsRPB1, Pol II, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2
Calculated Molecular Weight 217 kDa
Observed Molecular Weight 210-250 kDa
GenBank Accession NumberNM_000937
Gene Symbol POLR2A
Gene ID (NCBI) 5430
RRIDAB_2878716
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POLR2A antibody 20655-1-APDownload protocol
IHC protocol for POLR2A antibody 20655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Brahma-Related Gene 1 Deficiency in Endothelial Cells Ameliorates Vascular Inflammatory Responses in Mice.

Authors - Yuanyuan Zhang
View Article
humanWB,IF

J Exp Clin Cancer Res

LncMAPK6 drives MAPK6 expression and liver TIC self-renewal.

Authors - Guanqun Huang
View Article
humanWB

Cell Death Discov

Cezanne promoted autophagy through PIK3C3 stabilization and PIK3C2A transcription in lung adenocarcinoma

Authors - Yadong Wang
  • KD Validated
View Article