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验证数据展示

  • WB analysis using 15923-1-AP

    WB analysis using 15923-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15923-1-AP (POLR1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 15923-1-AP

    IHC staining of human ovary using 15923-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human ovary using 15923-1-AP

    IHC staining of human ovary using 15923-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15923-1-AP (POLR1C antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of NIH/3T3 using 15923-1-AP

    IP experiment of NIH/3T3 using 15923-1-AP

    IP result of anti-POLR1C (IP:15923-1-AP, 4ug; Detection:15923-1-AP 1:800) with NIH/3T3 cells lysate 1600 ug.

  • IHC staining of mouse liver using 15923-1-AP

    IHC staining of mouse liver using 15923-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15923-1-AP (POLR1C antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15923-1-AP

    IHC staining of mouse liver using 15923-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15923-1-AP (POLR1C antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

POLR1C Polyclonal antibody

POLR1C Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 15923-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

AC40, POLR1C, POLR1E, RPA39, RPA40, RPA5, RPAC1, RPC40

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells, HEK-293 cells, Caco-2 cells, HeLa cells, HepG2 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman ovary tissue, mouse liver tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human liver tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15923-1-AP targets POLR1C in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag8746

Product name: Recombinant human POLR1C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-346 aa of BC008863

Sequence: MAASQAVEEMRSRVVLGEFGVRNVHTTDFPGNYSGYDDAWDQDRFEKNFRVDVVHMDENSLEFDMVGIDAAIANAFRRILLAEVPTMAVEKVLVYNNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEEGTEIDTLQFRLQVRCTRNPHAAKDSSDPNELYVNHKVYTRHMTWIPLGNQADLFPEGTIRPVHDDILIAQLRPGQEIDLLMHCVKGIGKDHAKFSPVATASYRLLPDITLLEPVEGEAAEELSRCFSPGVIEVQEVQGKKVARVANPRLDTFSREIFRNEKLKKVVRLARVRDHYIFSVESTGVLPPDVLVSEAIKVLMGKCRRFLDELDAVQMD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (RNA) I polypeptide C, 30kDa
别名 AC40, POLR1C, POLR1E, RPA39, RPA40, RPA5, RPAC1, RPC40
计算分子量 346 aa, 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC008863
基因名称 POLR1C
Gene ID (NCBI) 9533
RRIDAB_2167328
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15160
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLR1C is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is a common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).

实验方案

Product Specific Protocols
WB protocol for POLR1C antibody 15923-1-APDownload protocol
IHC protocol for POLR1C antibody 15923-1-APDownload protocol
IP protocol for POLR1C antibody 15923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Genome Biol

Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture

Authors - Yongpeng Jiang
View Article
humanWB

Biochim Biophys Acta Mol Cell Res

POLR1D, a shared subunit of RNA polymerase I and III, modulates mTORC1 activity

Authors - Neuton Gorjão
View Article