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POLI Polyclonal antibody

POLI Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13635-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IP, IHC, ELISA and More (1)

DNA polymerase iota, Eta2, POLI, polymerase (DNA directed) iota, RAD30 homolog B, RAD30B, RAD3OB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse testis tissue, human heart tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13635-1-AP targets POLI in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, mosquito
免疫原 POLI fusion protein Ag4563 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed) iota
别名 DNA polymerase iota, Eta2, POLI, polymerase (DNA directed) iota, RAD30 homolog B, RAD30B, RAD3OB
计算分子量 715 aa, 80 kDa
观测分子量 87 kDa
GenBank蛋白编号BC032662
基因名称 POLI
Gene ID (NCBI) 11201
RRIDAB_2167009
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNA4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian Pol ι owns an unusual combination of properties: it is stimulated by Mn2+ ions, can bypass some DNA lesions and misincorporates "G" opposite template "T" more frequently than incorporates the correct "A." [PMID:21304950] It also was shown to possess dRP lyase activity. In addition, Pol ι is much more efficient in the presence of Mn2+ in comparison to Mg2+. [PMID:17609217]. It has been proposed that POLI involves in immunoglobulin somatic hypermutation (SHM), bypass of deaminated cytosines, several adducts of the purine bases. DNA strand crosslinks and is involved in DNA repair under oxidative stress[PMID:15199127].

实验方案

Product Specific Protocols
WB protocol for POLI antibody 13635-1-APDownload protocol
IHC protocol for POLI antibody 13635-1-APDownload protocol
IP protocol for POLI antibody 13635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Cell Death Dis

DNA polymerase iota promotes EMT and metastasis of esophageal squamous cell carcinoma by interacting with USP7 to stabilize HIF-1α

Authors - Aidi Gao
mouseWB

Oncogene

The error-prone DNA polymerase ι provides quantitative resistance to lung tumorigenesis and mutagenesis in mice.

Authors - M Iguchi
humanWB

Front Oncol

DNA Polymerase Iota Promotes Esophageal Squamous Cell Carcinoma Proliferation Through Erk-OGT-Induced G6PD Overactivation.

Authors - Zhenzi Su
  • KD Validated
humanWB,IHC

Cancer Sci

Phosphorylation of ETS-1 is a critical event in DNA polymerase iota-induced invasion and metastasis of esophageal squamous cell carcinoma.

Authors - Chao He
mosquitoIF

Insect Mol Biol

Diverse cellular morphologies during lumen maturation in Anopheles gambiae larval salivary glands.

Authors - M Chiu
humanIHC, IF

Int J Clin Exp Pathol

Elevated DNA polymerase iota (Poli) is involved in the acquisition of aggressive phenotypes of human esophageal squamous cell cancer.

Authors - Haoyao Sun
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