验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, H9C2 cells, mouse colon tissue, rat colon tissue, COLO 320 cells, A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85852-4-RR targets POLG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | POLG2 fusion protein Ag1424 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | polymerase (DNA directed), gamma 2, accessory subunit |
| 别名 | DNA polymerase subunit gamma-2, MTPOLB, p55, PEOA4, POLGB |
| 计算分子量 | 55 kDa |
| 观测分子量 | 52-55 kDa |
| GenBank蛋白编号 | BC009194 |
| 基因名称 | POLG2 |
| Gene ID (NCBI) | 11232 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UHN1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
by POLG) and an accessory subunit (encoded by POLG2). POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities (PMID: 37085601). Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) (PMID: 16685652).Western blot result suggested that the POLG2 expressed in cell lines with a single band of 52-55kd.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for POLG2 antibody 85852-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |