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验证数据展示

  • WB analysis using 85852-4-RR (same clone as 85852-4-PBS)

    WB analysis using 85852-4-RR (same clone as 85852-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85852-4-RR (POLG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85852-4-PBS in a different storage buffer formulation.

  • WB analysis using 85852-4-RR (same clone as 85852-4-PBS)

    WB analysis using 85852-4-RR (same clone as 85852-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85852-4-RR (POLG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85852-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85852-4-RR

    Affinity and Kinetic Characterization of 85852-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85852-4-RR against Human POLG2 were performed. The affinity constant is below 1 pM.

POLG2 Recombinant monoclonal antibody, PBS Only

POLG2 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85852-4-PBS

产品说明书

CloneNo. 250198G10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

DNA polymerase subunit gamma-2, MTPOLB, p55, PEOA4, POLGB

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85852-4-PBS targets POLG2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1424

Product name: Recombinant human POLG2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 136-485 aa of BC009194

Sequence: GPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPAYLETMQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polymerase (DNA directed), gamma 2, accessory subunit
别名 DNA polymerase subunit gamma-2, MTPOLB, p55, PEOA4, POLGB
计算分子量 55 kDa
观测分子量52-55 kDa
GenBank蛋白编号BC009194
基因名称 POLG2
Gene ID (NCBI) 11232
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHN1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

by POLG) and an accessory subunit (encoded by POLG2). POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities (PMID: 37085601). Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) (PMID: 16685652).Western blot result suggested that the POLG2 expressed in cell lines with a single band of 52-55kd.

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