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验证数据展示

  • IHC staining of human colon cancer using 10997-2-AP (same clone as 10997-2-PBS)

    IHC staining of human colon cancer using 10997-2-AP (same clone as 10997-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10997-2-AP (POLG2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

  • WB analysis of COLO 320 using 10997-2-AP (same clone as 10997-2-PBS)

    WB analysis of COLO 320 using 10997-2-AP (same clone as 10997-2-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 10997-2-AP (POLG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 10997-2-AP (same clone as 10997-2-PBS)

    IF Staining of U-251 using 10997-2-AP (same clone as 10997-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using POLG2 antibody (10997-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10997-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

POLG2 Polyclonal antibody, PBS Only

POLG2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10997-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DNA polymerase subunit gamma-2, MTPOLB, p55, PEOA4, POLGB

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10997-2-PBS targets POLG2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1424

Product name: Recombinant human POLG2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 136-485 aa of BC009194

Sequence: GPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPAYLETMQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed), gamma 2, accessory subunit
别名 DNA polymerase subunit gamma-2, MTPOLB, p55, PEOA4, POLGB
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC009194
基因名称 POLG2
Gene ID (NCBI) 11232
RRIDAB_2299887
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHN1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

mtDNA is replicated by DNA polymerase gamma, which is composed of a catalytic (encoded by POLG) and an accessory subunit (encoded by POLG2). POLG2(DNA polymerase subunit gamma-2, mitochondrial) can stimulate the polymerase and exonuclease activities (PMID: 37085601). Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4) (PMID: 16685652).Western blot result suggested that the POLG2 expressed in human fetal kidney and U-251 MG cell lines with a single band of 55kd.

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