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验证数据展示

  • WB analysis using 83670-1-RR (same clone as 83670-1-PBS)

    WB analysis using 83670-1-RR (same clone as 83670-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83670-1-PBS in a different storage buffer formulation.

  • WB analysis of rat testis using 83670-1-RR (same clone as 83670-1-PBS)

    WB analysis of rat testis using 83670-1-RR (same clone as 83670-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83670-1-PBS in a different storage buffer formulation.

  • WB analysis of rat brain using 83670-1-RR (same clone as 83670-1-PBS)

    WB analysis of rat brain using 83670-1-RR (same clone as 83670-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 83670-1-RR (POLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83670-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83670-1-RR

    Affinity and Kinetic Characterization of 83670-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83670-1-RR against Human POLG were performed. The affinity constant is below 1 pM.

POLG Recombinant monoclonal antibody, PBS Only

POLG Uni-rAb® Recombinant Antibody for ELISA, WB
Cat No. 83670-1-PBS

产品说明书

CloneNo. 240441D2

宿主/亚型

Rabbit / IgG

种属反应性

rat, human

应用

ELISA, WB

别名

EC:3.1.11.-, EC:2.7.7.7, DNA polymerase subunit gamma-1, 5'-deoxyribose-phosphate lyase, 3'-5' exodeoxyribonuclease

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83670-1-PBS targets POLG in ELISA, WB applications and shows reactivity with rat, human samples.

经测试应用 ELISA, WB Application Description
经测试反应性 rat, human
免疫原

CatNo: Ag5256

Product name: Recombinant human POLG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 888-1239 aa of BC042571

Sequence: GADVDSQELWIAAVLGDAHFAGMHGCTAFGWMTLQGRKSRGTDLHSKTATTVGISREHAKIFNYGRIYGAGQPFAERLLMQFNHRLTQQEAAEKAQQMYAATKGLRWYRLSDEGEWLVRELNLPVDRTEGGWISLQDLRKVQRETARKSQWKKWEVVAERAWKGGTESEMFNKLESIATSDIPRTPVLGCCISRALEPSAVQEEFMTSRVNWVVQSSAVDYLHLMLVAMKWLFEEFAIDGRFCISIHDEVRYLVREEDRYRAALALQITNLLTRCMFAYKLGLNDLPQSVAFFSAVDIDRCLRKEVTMDCKTPSNPTGMERRYGIPQGEALDIYQIIELTKGSLEKRSQPGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polymerase (DNA directed), gamma
别名 EC:3.1.11.-, EC:2.7.7.7, DNA polymerase subunit gamma-1, 5'-deoxyribose-phosphate lyase, 3'-5' exodeoxyribonuclease
计算分子量 140 kDa
GenBank蛋白编号BC042571
基因名称 POLG
Gene ID (NCBI) 5428
RRIDAB_3671275
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP54098
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POLG (DNA polymerase gamma) is composed of a C-terminal polymerase ('pol') domain and an amino-terminal exonuclease ('exo') domain. The exo domain increases the fidelity of mitochondrial DNA replication by conferring a proofreading activity to the enzyme (PMID: 12210792). Catalytic subunit of POLG solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates (PMID:11477093; 11897778; 15917273; 19837034; 9558343).

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